Analytical laboratory

The chemical laboratory of the State Institute of Agricultural Engineering and Bioenergy

In the chemical laboratory of the State Institute for Agricultural Engineering and Bioenergy, extensive chemical analyses are continuously carried out as part of a wide variety of projects. Modern laboratory equipment such as a gas chromatograph (GC), a high-pressure liquid chromatograph (HPLC), a high-performance analyzer for the simultaneous analysis of total carbon (TC), inorganic carbon (TIC) and total nitrogen (TN) in aqueous samples plus the TC content in solid samples, a decomposition and distillation system for the determination of total nitrogen and ammonium nitrogen in both aqueous and solid samples using the Kjeldahl method are used. In addition, the laboratory equipment is continuously being expanded and new methods for the chemical analysis of various parameters such as volatile fatty acids, sugars, alcohols, macro- and micronutrients, heavy metals, etc. are being (further) developed simultaneously. The most recent new development is the development of various methods for determining relevant chemical elements that are important for understanding and assessing the supply of nutrients and trace elements in various fermentation processes. In the meantime, the concentration of most of the elements of interest to the work of the state institute, e.g: Phosphorus (P), potassium (K), magnesium (Mg), sodium (Na), nickel (Ni), cobalt (Co), manganese (Mn), copper (Cu) and zinc (Zn) can be determined from liquid and solid samples. Depending on the problem, it is also possible to analyze only the heavy metal content of samples, e.g. lead and cadmium. All analysis results lead to a better understanding of process-biological fermentation procedures in ensiling processes, for the optimization of biogas production and/or for the biological production of platform chemicals within the framework of various projects. The following table provides an overview of selected analyses.




Parameter
Method and Device(s)
Description
Volatile Fatty AcidsCapillary Gas Chromatography (GC) - Varian GC (Type: CP-3800) with FID Detector and Varian CP 7777 Capillary Column (50m Length, 0.32 µm Diameter, 0.25mm Coating)Qualification and quantification of lower carboxylic acids from liquid and solid samples; e.g., acetic, propionic, n- and iso-butyric, n- and iso-valeric, caproic acid
Alcohols, SugarsHigh-Performance Liquid Chromatography (HPLC) - Bischoff device with RI Detector and a. BioRad Aminex HPLC Column HPX-87H (7.8 x 300mm; part. Size: 5.0µm) and BioRad Pre-column HPX-87H b. Hyperchrome HPLC Column Repro-Gel Ca (300 x 8.0mm; part. Size: 9.0µm)Qualification and quantification of various acids, sugars, and alcohols; e.g., DL-lactic acid, sucrose, fructose, glucose, ethanol, 1,2-propanediol, and mannitol
TC / TNThermo-catalytic High-Temperature Oxidation with Analytik Jena multi N/C 2100 SQuantification of inorganic carbon content as well as total carbon and nitrogen content in liquid samples; Quantification of total carbon content in solids
ICP-MSMass Spectrometry - Perkin Elmer ICP-MS NexION 2000Physical standard method for qualitative and quantitative determination of elemental contents. Inorganic element analysis for mass spectrometric trace analysis of, for example, heavy metals and micronutrients
CSB (Chemical Oxygen Demand)Photometry - LCK 014, 514 Cuvette Test by Dr. Lange, Thermostat LT 200 – Hach Lange; LASA 20 Sensor Array Photometer by Dr. LangeParameter indicating the degree of organic load in a liquid, determined according to a standardized procedure.
FOS / TACTitration with Metrohm tiamo 1.2, Titrando, and Sample processorParameter for determining the ratio of volatile organic acids (FOS) to the acid-binding capacity of the sample (TAC)
NH4-N (Ammonium Nitrogen)Distillation and Titration with Gerhardt Vapodest 50sAmmonia (NH3) and ammonium (NH4+) are in equilibrium (NH3+H2ONH4++OH-). They are captured together during the analysis (distillation followed by titration). The result can be given as ammonium concentration or, only referring to nitrogen, as ammonium nitrogen.
TKN (Kjeldahl Nitrogen)Acid digestion with Gerhardt digestion block with Turbosog and subsequent distillation and titration with Gerhardt Vapodest 50sSum of organically bound nitrogen and NH4-N as a measure of unoxidized nitrogen. In contrast to TN determination (see below), this method does not capture nitrogen from nitrite and nitrate compounds.